Isolation and diagnosis of some associated fungi with cowpea root rot disease and testing its pathogenicity

Execute search by date 1/4/2021, The results of collecting samples from the regions Anbar, Baghdad, Salah al-Din and Wasit showed that cowpea root rot disease is widespread in all studied areas, isolation phenotypic molecular diagnosis presence different fungi infected root, such as Fusarium nygamai (Fu1), F. (Fu2), solani (Fu3), solani(Fu4), Rhizoctonia (Rh5), (Fu6), pathogenicity test on radish seeds tested isolates were significantly decreased germination percentage water Ager, most effective was (Fu4). infection rate 90% compared to 0.00% control media treatment, which uncontaminated pathogenic fungus. Isolated a difference severity seedlings seedlings, isolate F.nygamai (Fu1) achieved highest 66.67 % at 75%. All fungal increased seed with treatment not contaminated 0.0%. Keywords: Vegan unguiculata; solani; PCR.